SARS-CoV-2 Nucleocapsid Protein Mouse Monoclonal Antibody [A4D12]
cat.: EM1902-21
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | SARS-CoV-2 |
| Applications: | ELISA, WB |
| Clonality: | Monoclonal |
| Clone number: | A4D12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 46 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant SARS-CoV-2 Nucleocapsid Protein. |
| Positive control: | ELISA: Recombinant SARS-CoV-2 Nucleocapsid Protein. WB: N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein and inactivated native SARS-CoV-2 lysate. |
| Subcellular location: | Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus. |
| Recommended Dilutions:
ELISA WB |
Use at an assay dependent concentration. 1:500-1:5,000 |
| Uniprot #: | SwissProt: P0DTC9 Gene ID: 1805293653 |
| Alternative names: | SARS-CoV-2 Nucleocapsid Protein SARS-CoV-2 NP nucleocapsid protein novel coronavirus N Protein novel coronavirus Nucleocapsid Protein 2019-nCoV Nucleoprotein 2019-nCoV N 2019nCoV N 2019-nCoV N Protein 2019 peak N Protein 2019-nCoV nucleocapsid protein |
Images
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Fig1:
The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein. Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml. |
|
Fig2:
Western blot analysis of EM1902-21 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.