|Product Type:||Mouse monoclonal IgG1, primary antibodies|
The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.
Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml.
Western blot analysis of EM1902-21 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.