PAX8 Mouse Monoclonal Antibody [6G1]
cat.: EM1902-22
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 6G1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within human PAX8 aa 400-450.
Positive control: 293T cell, 293.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:200-1:1,000
1:50-1:100
Uniprot #: SwissProt: Q06710 Human
Alternative names: OTTHUMP00000158659 OTTHUMP00000158660 OTTHUMP00000203723 OTTHUMP00000203724 Paired box 8 Paired box gene 8 paired box homeotic gene 8 Paired box protein Pax 8 Paired box protein Pax-8 Paired domain gene 8 PAX 8 PAX8 PAX8_HUMAN
Images
EM1902-22_1.jpg Fig1: Western blot analysis of PAX8 on 293T cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1902-22_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-PAX8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-22_3.jpg Fig3: Flow cytometric analysis of PAX8 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.