GATA3 Mouse Monoclonal Antibody [A3G6]
cat.: EM1902-23
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: A3G6
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 48 kDa.
Isotype: IgG1
Immunogen: Synthetic peptide corresponding to N terminal of Human GATA3.
Positive control: MCF-7 cell lysates.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: P23771 Human
Alternative names: GATA 3 GATA binding factor 3 GATA binding protein 3 GATA-binding factor 3 Gata3 GATA3_HUMAN HDR HDRS MGC2346 MGC5199 MGC5445 Trans acting T cell specific transcription factor GATA 3 Trans-acting T-cell-specific transcription factor GATA-3
Images
EM1902-23_1.jpg Fig1: Western blot analysis of GATA3 on different lysates with Rabbit anti-GATA3 antibody (EM1902-23) at 1/1,000 dilution.

Lane 1:SH-SY5Y(Human neuroblastoma cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 60 seconds ; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1902-23, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 48 kDa
Observed band size: 48 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.