GADD34 Mouse Monoclonal Antibody [A4D7]
cat.: EM1902-26
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A4D7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human GADD34 aa 1-50 / 674. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, K562 cell lysate, human prostate carcinoma tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: O75807 Human |
| Alternative names: | Apoptosis associated protein GADD 34 Growth arrest and DNA damage inducible 34 Growth arrest and DNA damage-inducible protein GADD34 Myeloid differentiation primary response protein MyD116 homolog Ppp1r15a PR15A_HUMAN Protein phosphatase 1 regulatory (inhibitor) subunit 15A Protein phosphatase 1 regulatory subunit 15A |
Images
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Fig1:
Western blot analysis of GADD34 on different lysates with Mouse anti-GADD34 antibody (EM1902-26) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si GADD34 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 73 kDa Observed band size: 65 kDa Exposure time: 2 minutes 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of GADD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-GADD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.