GADD34 Mouse Monoclonal Antibody [A4D7]
cat.: EM1902-26
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A4D7
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 73 kDa
Isotype: IgG1
Immunogen: Synthetic peptide within Human GADD34 aa 1-50 / 674.
Positive control: HepG2 cell lysate, K562 cell lysate, human prostate carcinoma tissue, K562.
Subcellular location: Endoplasmic reticulum membrane, Mitochondrion outer membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O75807 Human
Alternative names: Apoptosis associated protein GADD 34 Growth arrest and DNA damage inducible 34 Growth arrest and DNA damage-inducible protein GADD34 Myeloid differentiation primary response protein MyD116 homolog Ppp1r15a PR15A_HUMAN Protein phosphatase 1 regulatory (inhibitor) subunit 15A Protein phosphatase 1 regulatory subunit 15A
Images
EM1902-26_1.jpg Fig1: Western blot analysis of GADD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
EM1902-26_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-GADD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-26_3.jpg Fig3: Flow cytometric analysis of GADD34 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-26, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.