Myoglobin Mouse Monoclonal Antibody [A4B7]
cat.: EM1902-27
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A4B7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Myoglobin aa 1-140 / 154. |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, rat heart tissue, human fetal skeletal muscle tissue. |
| Subcellular location: | Cytosol, extracellular exosome. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000-1:5,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P02144 Human | P04247 Mouse | Q9QZ76 Rat |
| Alternative names: | MB MGC13548 MYG_HUMAN Myoglobin |
Images
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Fig1:
Western blot analysis of Myoglobin on different lysates with Mouse anti-Myoglobin antibody (EM1902-27) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lane 3: Mouse heart tissue lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunofluorescence staining of paraffin- embedded human fetal skeletal muscle tissue using anti-Myoglobin antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with EM1902-27 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Mouse IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myoglobin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-27, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Myoglobin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-27, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.