ZAP70 Mouse Monoclonal Antibody [A1B7]
cat.: EM1902-28
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A1B7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human ZAP70 aa 200-500. |
| Positive control: | Jurkat cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate, LOVO, Jurkat. |
| Subcellular location: | Cell membrane, cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: P43403 Human | P43404 Mouse Entrez Gene: 301348 Rat |
| Alternative names: | 70 kDa zeta associated protein 70 kDa zeta-associated protein EC 2.7.10.2 FLJ17670 FLJ17679 Selective T cell defect SRK STD Syk related tyrosine kinase Syk-related tyrosine kinase Truncated ZAP kinase Tyrosine protein kinase ZAP70 Tyrosine-protein kinase ZAP-70 TZK ZAP 70 ZAP70 ZAP70_HUMAN Zeta chain associated protein kinase 70kD Zeta chain associated protein kinase 70kDa Zeta chain associated protein kinase 70kDa isoform 1 Zeta chain associated protein kinase 70kDa isoform 2 Zeta chain of T cell receptor associated protein kinase 70 Zeta chain TCR associated protein kinase 70kD Zeta chain TCR associated protein kinase 70kDa |
Images
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Fig1:
Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (EM1902-28) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of ZAP70 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1902-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of ZAP70 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-28, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.