CD79a Mouse Monoclonal Antibody [C1-E8]
cat.: EM1902-29
| Product Type: | Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | C1-E8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse CD79a aa 100-200. |
| Positive control: | Rat spleen tissue lysates, Daudi. |
| Subcellular location: | Cell membrane, Membrane |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P11912 Human | P11911 Mouse | 100913063 Rat |
| Alternative names: | B lymphocyte-specific MB1 protein B-cell antigen receptor complex-associated protein alpha chain CD 79a CD79a CD79a antigen (immunoglobulin-associated alpha) CD79A antigen CD79a molecule, immunoglobulin-associated alpha CD79A_HUMAN Ig alpha Ig-alpha IGA IgM-alpha Immunoglobulin-associated alpha Ly54 MB-1 membrane glycoprotein MB1 Membrane-bound immunoglobulin-associated protein Surface IgM-associated protein |
Images
|
Fig1:
Western blot analysis of CD79a on rat spleen tissue lysates with Mouse anti-CD79a antibody (EM1902-29) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 25 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of CD79a was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-29, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.