CD79a Mouse Monoclonal Antibody [C1-E8]
cat.: EM1902-29
Product Type: Mouse monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, FC
Clonality: Monoclonal
Clone number: C1-E8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/ml.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size 25 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within mouse CD79a aa 100-200.
Positive control: Human thymus tissue lysate, mouse spleen tissue lysate, Daudi cell lysate.
Subcellular location: Cell membrane, Membrane
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P11912 Human | P11911 Mouse
Alternative names: B lymphocyte-specific MB1 protein B-cell antigen receptor complex-associated protein alpha chain CD 79a CD79a CD79a antigen (immunoglobulin-associated alpha) CD79A antigen CD79a molecule, immunoglobulin-associated alpha CD79A_HUMAN Ig alpha Ig-alpha IGA IgM-alpha Immunoglobulin-associated alpha Ly54 MB-1 membrane glycoprotein MB1 Membrane-bound immunoglobulin-associated protein Surface IgM-associated protein
Images
EM1902-29_1.jpg Fig1: Western blot analysis of CD79a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human thymus tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Daudi cell lysate
EM1902-29_2.jpg Fig2: Flow cytometric analysis of CD79a was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-29, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.