|Product Type:||Mouse monoclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse|
Western blot analysis of CD79a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Human thymus tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Daudi cell lysate
|Fig2: Flow cytometric analysis of CD79a was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-29, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).|