Anti-APOER2 antibody [13B2]
cat.: EM1902-30
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: WB, ICC
Clonality: Monoclonal
Clone number: 13B2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/ml.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 106/78/99/87 kDa.
Isotype: IgG2a
Immunogen: Recombinant protein within Human APOER2 aa 47-275 / 963.
Positive control: A549 cell lysate, SHSY5Y cell lysate, SKBR-3 cell lysate, U937 cell lysate, SKOV-3.
Subcellular location: Cell membrane, Membrane, Secreted.
Recommended Dilutions:
  WB
  ICC

1:500
1:50
Uniprot #: SwissProt: Q14114 Human
Alternative names: APOER2 Apolipoprotein E receptor 2 low density lipoprotein receptor-related protein 8 Low-density lipoprotein receptor-related protein 8 LRP-8 LRP8 LRP8_HUMAN
Images
EM1902-30_1.jpg Fig1: Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: SHSY5Y cell lysate
Lane 3: SKBR-3 cell lysate
Lane 4: U937 cell lysate
EM1902-30_2.jpg Fig2: ICC staining of APOER2 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1902-30, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.