APOER2 Mouse Monoclonal Antibody [13B2]
cat.: EM1902-30
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 13B2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within Human APOER2 aa 47-275 / 963. |
| Positive control: | A549 cell lysate, SHSY5Y cell lysate, SKBR-3 cell lysate, U937 cell lysate, Hela, A549, human testis tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: Q14114 Human |
| Alternative names: | APOER2 Apolipoprotein E receptor 2 low density lipoprotein receptor-related protein 8 Low-density lipoprotein receptor-related protein 8 LRP-8 LRP8 LRP8_HUMAN |
Images
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Fig1:
Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: SHSY5Y cell lysate Lane 3: SKBR-3 cell lysate Lane 4: U937 cell lysate |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-APOER2 antibody (EM1902-30) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-30) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.