YKL-40 / CHI3L1 Mouse Monoclonal Antibody [A3G10]
cat.: EM1902-31
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A3G10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within extracellular domain of Human CHI3L1. |
| Positive control: | THP-1 (Human acute monoblastic leukemia cell) cell lysate, U-87 MG (Human glioblastoma cell) cell lysate, human tonsil tissue. |
| Subcellular location: | Extracellular space, Endoplasmic reticulum, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:50-1:200 |
| Uniprot #: | SwissProt: P36222 Human |
| Alternative names: | 39 kDa synovial protein ASRT7 Cartilage glycoprotein 39 CGP-39 CGP39 CH3L1_HUMAN CHI3L1 chitinase 3 like 1 (cartilage glycoprotein 39) chitinase 3 like 1 Chitinase 3 like protein 1 precursor chitinase Chitinase-3-like protein 1 Chondrocyte protein YKL40 GP 39 GP-39 GP39 HC gp39 HCGP 3P hCGP-39 HCgp39 YKL 40 YKL-40 YKL40 YYL 40 |
Images
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Fig1:
Western blot analysis of YKL-40 / CHI3L1 on different lysates with Mouse anti-YKL-40 / CHI3L1 antibody (EM1902-31) at 1/5,000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 3: U-87 MG (Human glioblastoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Jurkat is a negative control (PMID: 16472595). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1902-31, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43 kDa Observed band size: 40 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-YKL-40 / CHI3L1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-31, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.