MUC1 Mouse Monoclonal Antibody [A4D9]
cat.: EM1902-32
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: A4D9
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size 122 kDa.
Isotype: IgG1
Immunogen: Synthetic peptide of core peptide domain of human MUC1.
Positive control: Human breast tissue, human kidney tissue, human uterus tissue, human colon carcinoma tissue, human breast carcinoma tissue, MCF-7, HeLa.
Subcellular location: Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.
Recommended Dilutions:
  IHC-P
  FC
  IF-Cell
1:50-1:500
1:50-1:100
1:500
Uniprot #: SwissProt: P15941 Human
Alternative names: ADMCKD ADMCKD1 Breast carcinoma associated antigen DF3 Breast carcinoma-associated antigen DF3 CA 15-3 CA15 3 CA15 3 antigen CA15-3 CA15.3 Cancer antigen 15-3 Carcinoma associated mucin Carcinoma-associated mucin CD 227 CD227 DF3 antigen EMA Episialin Epithelial Membrane Antigen H23 antigen H23AG KL 6 KL-6 KL6 Krebs von den Lungen-6 MAM 6 MAM6 MCD MCKD MCKD1 Medullary cystic kidney disease 1 (autosomal dominant) Medullary cystic kidney disease, autosomal dominant MUC 1 MUC-1 MUC-1/SEC MUC-1/X MUC1 MUC1-alpha MUC1-beta MUC1-CT MUC1-NT MUC1/ZD MUC1_HUMAN Mucin 1 Mucin 1 cell surface associated Mucin 1 transmembrane Mucin 1, cell surface associated Mucin-1 subunit beta Peanut reactive urinary mucin Peanut-reactive urinary mucin PEM PEMT Polymorphic epithelial mucin PUM Tumor associated epithelial membrane antigen Tumor associated epithelial mucin Tumor associated mucin Tumor-ass......
Images
EM1902-32_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-32, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-32_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-32, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-32_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-32, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-32_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-32_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-32_6.jpg Fig6: Flow cytometric analysis of MUC1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-32, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
EM1902-32_7.jpg Fig7: Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) labeling MUC1 with Mouse anti-MUC1 antibody (EM1902-32) at 1/500 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MUC1 antibody (EM1902-32) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.