Anti-GATA3 antibody [A3G3]
cat.: EM1902-33
Product Type: Mouse monoclonal IgG2c, primary antibodies
Species reactivity: Human
Applications: WB, ICC, FC
Clonality: Monoclonal
Clone number: A3G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa.
Isotype: IgG2c
Immunogen: Synthetic peptide within N-terminal Human GATA3.
Positive control: MCF-7 cell lysates, MCF-7, jurkat.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P23771 Human
Alternative names: GATA 3 GATA binding factor 3 GATA binding protein 3 GATA-binding factor 3 Gata3 GATA3_HUMAN HDR HDRS MGC2346 MGC5199 MGC5445 Trans acting T cell specific transcription factor GATA 3 Trans-acting T-cell-specific transcription factor GATA-3
Images
EM1902-33_1.jpg Fig1: Western blot analysis of GATA3 on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Specific bands were detected for GATA3 full length (FL) at approximately 52 kDa and the splice form (SF) at approximately 39 kDa (as indicated).
EM1902-33_2.jpg Fig2: ICC staining of GATA3 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1902-33, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
EM1902-33_3.jpg Fig3: Flow cytometric analysis of GATA3 was done on jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-33, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.