FOXA1 Mouse Monoclonal Antibody [A2E8]
cat.: EM1902-34
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: A2E8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 49 kDa.
Isotype: IgG1
Immunogen: Synthetic peptide within N-terminal human FOXA1.
Positive control: LNCaP cell lysates, LNCaP, human prostate tissue, MCF-7.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell

1:2,000
1:500
1:50-1:100
1:100
Uniprot #: SwissProt: P55317 Human
Alternative names: forkhead box A1 Forkhead box protein A1 FOX A1 FOXA1 FOXA1_HUMAN hepatocyte nuclear factor 3 alpha Hepatocyte nuclear factor 3-alpha HNF 3A HNF-3-alpha HNF-3A HNF3A MGC33105 TCF 3A TCF-3A TCF3A Transcription factor 3A
Images
EM1902-34_1.jpg Fig1: Western blot analysis of FOXA1 on LNCaP cell lysates with Mouse anti-FOXA1 antibody (EM1902-34) at 1/2,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 49 kDa
Observed band size: 49 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM1902-34_2.jpg Fig2: Immunocytochemistry analysis of LNCaP cells labeling FOXA1 with Mouse anti-FOXA1 antibody (EM1902-34) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-FOXA1 antibody (EM1902-34) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
EM1902-34_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human prostate tissue with Mouse anti-FOXA1 antibody (EM1902-34) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-34) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1902-34_4.jpg Fig4: Flow cytometric analysis of FOXA1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.