SIRP alpha Mouse Monoclonal Antibody [A3E3]
cat.: EM1902-37
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A3E3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human SIRP alpha aa 1-400. |
| Positive control: | SW480 cell lysates, THP-1, human lung cancer tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P78324 Human |
| Alternative names: | Signal regulatory protein alpha type 1 Bit Brain Ig like molecule with tyrosine based activation motifs Brain Ig-like molecule with tyrosine-based activation motifs Brain immunoglobulin like molecule with tyrosine based activation motifs CD172 antigen like family member A CD172 antigen-like family member A CD172a CD172a antigen Inhibitory receptor SHPS-1 Macrophage fusion receptor MFR MYD 1 Myd 1 antigen MyD-1 antigen p84 Protein tyrosine phosphatase non receptor type substrate 1 PTPNS1 SHP substrate 1 SHPS-1 SHPS1 SHPS1_HUMAN Signal regulatory protein alpha 2 Signal regulatory protein alpha 3 Signal regulatory protein alpha Signal regulatory protein alpha type 2 Signal-regulatory protein alpha-1 Signal-regulatory protein alpha-2 Signal-regulatory protein alpha-3 SIRP Sirp-alpha-1 Sirp-alpha-2 Sirp-alpha-3 SIRPA SIRPalpha SIRPalpha1 SIRPalpha2 SIRPalpha3 Tyrosine phosphatase SHP substrate 1 Tyrosine protein p...... |
Images
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Fig1: Western blot analysis of SIRP alpha on SW480 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of THP-1 cells labeling SIRP alpha with Mouse anti-SIRP alpha antibody (EM1902-37) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIRP alpha antibody (EM1902-37) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SIRP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-37, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of THP-1 cells labeling SIRP alpha. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (EM1902-37, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.