Myc tag Mouse Monoclonal Antibody [A3C8]
cat.: EM1902-38
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA, IP |
| Clonality: | Monoclonal |
| Clone number: | A3C8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin. |
| Positive control: | C-terminal Myc-tagged recombinant protein lysates, N-terminal Myc-tagged recombinant protein lysates. |
| Recommended Dilutions:
WB ELISA IP |
1:1,000-1:5,000 Use at an assay dependent concentration. 2-5 µg/ml. |
| Alternative names: | AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Cellular myelocytomatosis oncogene Class E basic helix-loop-helix protein 39 MGC105490 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 myca MYCC Myelocytomatosis oncogene a Myelocytomatosis oncogene Niard Nird oncogene c-Myc Oncogene Myc OTTHUMP00000158589 OTTHUMP00000227763 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) zc-myc |
Images
|
Fig1: Western blot analysis of Myc tag on C-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of Myc tag on N-terminal Myc-tagged recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-38, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:10,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immobilized N-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃. |
|
Fig4:
Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
|
Fig5:
Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM1902-38 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM1902-38 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.