CD55 Mouse Monoclonal Antibody [12F1]
cat.: EM1902-42
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: 12F1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/ml.
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 41 kDa.
Isotype: IgG1
Immunogen: Recombinant protein within human CD55 aa 150- 381.
Positive control: Hela, SW1990.
Subcellular location: Cell membrane, Membrane, Secreted.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P08174 Human
Alternative names: CD 55 CD55 CD55 antigen CD55 Cromer blood group system CD55 molecule (Cromer blood group) CD55 molecule CD55 molecule, decay accelerating factor for complement (Cromer blood group) Cd55a Complement decay accelerating factor Complement decay-accelerating factor Complement decay-accelerating factor, GPI-anchored CR CROM Cromer Blood Group antigen Cromer blood group system DAF Daf-GPI DAF_HUMAN Daf1 Dcay accelerating factor for complement (CD55, Cromer blood group system) Decay accelarating factor 1, isoform CRA_a Decay accelerating factor (GPI-form) Decay Accelerating Factor for Complement Decay accelerating factor GPI-form Decay accelerating factor soluble-form GPI-DAF TC
Images
EM1902-42_1.jpg Fig1: Western blot analysis of CD55 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
EM1902-42_2.jpg Fig2: Flow cytometric analysis of CD55 was done on SW1990 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-42, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.