CELF2 Mouse Monoclonal Antibody [9B1]
cat.: EM1902-43
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 9B1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2 mg/mL. |
| Purification: | Protein G affinity purified. |
| Molecular weight: | 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human CELF2 aa 460-508. |
| Positive control: | PC-3M cell lysates, rat skeletal muscle tissue, mouse colon tissue, Daudi. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O95319 Human | Q9Z0H4 Mouse | Q792H5 Rat |
| Alternative names: | Bruno-like protein 3 BRUNOL3 Cardiac elav type RNA binding protein CELF-2 celf2 CELF2_HUMAN CUG BP2 CUG triplet repeat RNA-binding protein 2 CUG-BP- and ETR-3-like factor 2 CUG-BP2 CUGBP Elav like family member 2 CUGBP Elav-like family member 2 CUGBP2 dJ323N1.1 elav type RNA binding protein ELAV-type RNA-binding protein 3 ETR 3 ETR-3 ETR3 HGNC:2550 hNAPOR NAPOR 2 NAPOR Neuroblastoma apoptosis-related RNA-binding protein Neuroplastoma apoptosis related RNA binding protein 3 RNA binding protein BRUNOL3 RNA-binding protein BRUNOL-3 |
Images
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Fig1: Western blot analysis of CELF2 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CELF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-43, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CELF2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1902-43, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of CELF2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1902-43, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.