TIMP-1 Mouse Monoclonal Antibody [4C2]
cat.: EM2001-03
Product Type: Mouse monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Tissue
Clonality: Monoclonal
Clone number: 4C2
Form: Liquid
Storage condition: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration: 1ug/ul
Purification: Protein G affinity purified.
Molecular weight: 21 kDa
Isotype: IgG
Immunogen: Recombinant human TIMP-1
Positive control: Human liver carcinoma tissue, Human brain glioma tissue, Mouse pancreas tissue, Human brain glioma tissue, Rat brain tissue, Mouse brain tissue, Human colon carcinoma tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-tissue
1:500-2000
1:100-500
1:200-800
Uniprot #: SwissProt: P01033 Human
Alternative names: Clgi Collagenase inhibitor Collagenase inhibitor Human EPA EPO Erythroid Potentiating Activity Erythroid-potentiating activity Fibroblast collagenase inhibitor FLJ90373 HCI Human Collagenase Inhibitor Metalloproteinase inhibitor 1 Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214 TIMP 1 TIMP TIMP metallopeptidase inhibitor 1 TIMP-1 Timp1 TIMP1 protein TIMP1_HUMAN Tissue Inhibitor of Metalloproteinase 1 Tissue inhibitor of metalloproteinases 1 Tissue inhibitor of metalloproteinases Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
Images
EM2001-03_1.jpg Fig1: Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Rat Stomach tissue lysates
Lane 4: Rat Colon tissue lysates
Lane 5: Rat Lung tissue lysates
Primary: Anti-TIMP-1 (bsm-10895M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 28 kDa
EM2001-03_2.jpg Fig2: Sample:
Recombinant human TIMP-1 Protein at 40 ug
Primary: Anti-TIMP-1 (bsm-10895M) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 19 kD
EM2001-03_3.jpg Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_4.jpg Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_5.jpg Fig5: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (bsm-10895M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
EM2001-03_6.jpg Fig6: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_7.jpg Fig7: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_8.jpg Fig8: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Polyclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_9.jpg Fig9: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
EM2001-03_10.jpg Fig10: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIMP-1) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.