Anti-Bmi1 antibody [B3-G5]
cat.: EM20602
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: B3-G5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A purified.
Molecular weight: 37kDa
Isotype: IgG2a
Immunogen: Recombinant protein within human Bmi1 full sequence.
Positive control: Hela, HepG2, Jurkat, 293T, MCF-7, PC12, K562, NIH/3T3, A549, LOVO, human tonsil tissue, human colon cancer tissue, human breast cancer tissue, mouse kidney tissue, human kidney tissue, human brain tissue.
Subcellular location: Nucleus, Cytoplasm
Recommended Dilutions:
  WB
  IHC-P
  ICC
  FC

1:1,000
1:200
1:200
1:100-1:200
Uniprot #: P35226(Human) P25916(Mouse)
Alternative names: B lymphoma Mo MLV insertion region (mouse) antibody B lymphoma Mo MLV insertion region 1 homolog antibody Bmi 1 antibody BMI1 antibody BMI1 polycomb ring finger oncogene antibody BMI1_HUMAN antibody Flvi 2/bmi 1 antibody FLVI2/BMI1 antibody MGC12685 antibody Murine leukemia viral (bmi 1) oncogene homolog antibody Oncogene BMI 1 antibody PCGF 4 antibody PCGF4 antibody Polycomb complex protein BMI 1 antibody Polycomb complex protein BMI-1 antibody Polycomb group protein Bmi1 antibody Polycomb group ring finger 4 antibody Polycomb group RING finger protein 4 antibody RING finger protein 51 antibody RNF 51 antibody RNF51 antibody
Images
EM20602_1.jpg Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Jurkat
Lane 3: Hela
Lane 4: MCF-7
Lane 5: HepG2
Lane 6: NIH/3T3
Lane 7: PC12
Lane 8: Mouse kidney
Lane 9: Human kidney
Lane 10: K562
Lane 11: Human brain
EM20602_2.jpg Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM20602_3.jpg Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM20602_4.jpg Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM20602_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
EM20602_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
EM20602_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
EM20602_8.jpg Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.