p53 Mouse Monoclonal Antibody [7-5]
cat.: EM20603
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 7-5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human p53 aa 50-393. |
| Positive control: | A431 cell lysate, 293 cell lysate, human colonic carcinoma tissue, human gastric carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:1,000-1:2,000 1:100-1:200 1:100-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1:
Western blot analysis of p53 on 293T (Human embryonic kidney cell) cell lysate with Rabbit anti-p53 antibody (EM20603) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM20603, 1/1,000 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
|
Fig2:
Western blot analysis of p53 on A431 (Human epidermoid carcinoma skin squamous cell) cell lysate with Rabbit anti-p53 antibody (EM20603) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM20603, 1/1,000 in 5% NFDM/TBST, 4° overnight Secondary antibody: Goat Anti-Mouse IgG-HRP(HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
|
Fig3: ICC staining p53 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.