p53 Mouse Monoclonal Antibody [7-5]
cat.: EM20603
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 7-5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human p53 aa 50-393. |
| Positive control: | A431 cell lysate, 293 cell lysate, A431, human colonic carcinoma tissue, human gastric carcinoma tissue. |
| Subcellular location: | Nucleus, Cytoplasm |
| Recommended Dilutions:
WB IHC-P FC IF-Cell |
1:1,000-1:2,000 1:100-1:200 1:100-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P04637 Human |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
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Fig1:
All lanes: Western blot analysis of p53 with anti-p53 antibody (EM20603) at 1:500 dilution. Lane 1/2: Wild-type A431 whole cell lysate (10 µg). Lane 3/4: p53 knockout A431 whole cell lysate (10 µg). EM20603 was shown to specifically react with p53 in wild-type A431 cells. No band was observed when p53 knockout sample was tested. Wild-type and p53 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM20603, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of p53 on different lysates with Mouse anti-p53 antibody (EM20603) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: 293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM20603) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining p53 in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-p53 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with p53 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.