HSP90 Beta Mouse Monoclonal Antibody [2-1-G3]
cat.: EM21103
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: 2-1-G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG2a
Immunogen: Synthetic peptide within Human HSP90 Beta aa 311-360 / 724.
Positive control: HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, human colon carcinoma tissue, human tonsil tissue, mouse testis tissue, mouse brain tissue.
Subcellular location: Cytoplasm
Recommended Dilutions:
  WB
  IHC-P
1:1,000-1:5,000
1:500-1:10,000
Uniprot #: SwissProt: P08238 Human | P11499 Mouse | P34058 Rat
Alternative names: 90 kda heat shock protein beta HSP90 beta D6S182 FLJ26984 Heat shock 84 kDa Heat shock 90kD protein 1, beta Heat shock 90kDa protein 1 beta Heat shock protein 90 alpha family class B member 1 Heat shock protein 90 kDa Heat shock protein 90kDa alpha (cytosolic) class B member 1 Heat shock protein 90kDa alpha family class B member 1 Heat shock protein beta Heat shock protein HSP 90 beta Heat shock protein HSP 90-beta HS90B_HUMAN HSP 84 HSP 90 HSP 90 b HSP 90b HSP84 HSP90 BETA hsp90ab1 HSP90B HSPC2 HSPCB
Images
EM21103_1.jpg Fig1: Western blot analysis of HSP90 Beta on different lysates with Mouse anti-HSP90 Beta antibody (EM21103) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Mouse heart tissue lysate (40 µg/Lane)
Lane 11: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 53 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
EM21103_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM21103_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-HSP90 Beta antibody (EM21103) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM21103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM21103_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
EM21103_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
EM21103_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
EM21103_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSP90 beta antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.