GFP Mouse Monoclonal Antibody [2-D8]
cat.: EM30501
Product Type: Mouse monoclonal IgG1, primary antibodies
Applications: WB, ICC, ELISA, IP, FC, IP
Clonality: Monoclonal
Clone number: 2-D8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 26 kDa
Isotype: IgG1
Immunogen: Recombinant full length protein of Aequorea victoria GFP.
Positive control: Transfected K562 with GFP.
Recommended Dilutions:
  WB
  ICC
  IP

1:2,000
1:200
2-5 µg/ml.
Uniprot #: SwissProt: P42212 Aequorea victoria
Alternative names: GFP Green fluorescent protein yfp
Images
EM30501_1.jpg Fig1: Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution.
EM30501_2.jpg Fig2: ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM30501_3.jpg Fig3: GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with EM30501 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: M1210-1 IP in GFP Tag fusion protein lysate.
Lane 3: EM30501 IP in GFP Tag fusion protein lysate.
Lane 4: Mouse IgG instead of EM30501 in GFP Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.