GFP Mouse Monoclonal Antibody [2-D8]
cat.: EM30501
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, ELISA, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | 2-D8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full length protein of Aequorea victoria GFP. |
| Positive control: | Transfected K562 with GFP. |
| Recommended Dilutions:
WB IF-Cell IP |
1:2,000 1:200 2-5 µg/ml. |
| Uniprot #: | SwissProt: P42212 Aequorea victoria |
| Alternative names: | GFP Green fluorescent protein yfp |
Images
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Fig1: Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution. |
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Fig2: ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3:
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with EM30501 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1604-25 at 1/1000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: M1210-1 IP in GFP Tag fusion protein lysate. Lane 3: EM30501 IP in GFP Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM30501 in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.