PRMT5 Mouse Monoclonal Antibody [7-C2]
cat.: EM31103
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7-C2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within Human PRMT5 aa 1-50 / 637. |
| Positive control: | Human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:100-1:200 |
| Uniprot #: | SwissProt: O14744 Human |
| Alternative names: | 72 kDa ICln binding protein 72 kDa ICln-binding protein ANM5_HUMAN Histone synthetic lethal 7, S. cerevisiae, homolog of Histone-arginine N-methyltransferase PRMT5 HMT1 hnRNP methyltransferase like 5 HOMOLOG OF; SKB1 HRMT1L5 IBP72 Jak-binding protein 1 JBP 1 JBP1 PRMT 5 PRMT5 Protein arginine methyltransferase 5 Protein arginine N methyltransferase 5 Protein arginine N methyltransferase 5 N terminally processed Protein arginine N-methyltransferase 5 S. POMBE S. POMBE HOMOLOG OF; SKB1 SHK1 KINASE BINDING PROTEIN 1 Shk1 kinase binding protein 1 homolog Shk1 kinase-binding protein 1 homolog Shk1 kinase/binding protein 1, S. pombe, homolog of SKB 1 SKB1 SKB1 homolog SKB1: SKB1 homolog (S. pombe) SKB1Hs |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin. |
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Fig7:
Western blot analysis of PRMT5 on different lysates with Mouse anti-PRMT5 antibody (EM31103) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissuel lysate Lysates/proteins at 40 µg/Lane. Exposure time: 2 minutes 18 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM31103, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 72.7 kDa Observed band size: 73 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.