Myc Tag Mouse Monoclonal Antibody [A3-B4]
cat.: EM31105
Product Type: Mouse monoclonal IgG2b, primary antibodies
Applications: WB, ICC, ELISA, IP, FC, IP
Clonality: Monoclonal
Clone number: A3-B4
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Isotype: IgG2b
Immunogen: Synthetic peptide immune sequence is EQKLISEEDLC-KLH.
Positive control: Myc-tagged recombinant protein.
Recommended Dilutions:
  WB
  IP

1:2,000
2-5 µg/ml.
Alternative names: avian myelocytomatosis viral oncogene homolog bHLHe39 c-Myc class E basic helix-loop-helix protein 39 MRTL MYC Myc Epitope Tag myc proto-oncogene protein myc-related translation/localization regulatory factor oncogene c-Myc proto-oncogene c-Myc protooncogene homologous to myelocytomatosis virus transcription factor p64 v-myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian)
Images
EM31105_1.jpg Fig1: Western blot analysis of Myc Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal Myc-tagged recombinant protein
Lane 2: N-terminal Myc-tagged recombinant protein
EM31105_2.jpg Fig2: ICC staining of Myc Tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM31105_3.jpg Fig3: Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

Lane 1: Myc Tag fusion protein lysate (input).
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
EM31105_4.jpg Fig4: Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.

Lane 1: Myc Tag fusion protein lysate (input).
Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate.
Lane 3: EM31105 IP in Myc Tag fusion protein lysate.
Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.