Myc tag Mouse Monoclonal Antibody [A3-B4]
cat.: EM31105
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, ELISA, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | A3-B4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide immune sequence is EQKLISEEDLC-KLH. |
| Positive control: | Myc-tagged recombinant protein. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:2,000 1:50 1:50-1:100 2-5 µg/ml. |
| Alternative names: | avian myelocytomatosis viral oncogene homolog bHLHe39 c-Myc class E basic helix-loop-helix protein 39 MRTL MYC Myc Epitope Tag myc proto-oncogene protein myc-related translation/localization regulatory factor oncogene c-Myc proto-oncogene c-Myc protooncogene homologous to myelocytomatosis virus transcription factor p64 v-myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) |
Images
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Fig1:
Western blot analysis of Myc tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM31105, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: C-terminal Myc-tagged recombinant protein Lane 2: N-terminal Myc-tagged recombinant protein |
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Fig2: ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM31105, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3:
Myc tag was immunoprecipitated in 2µg C terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
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Fig4:
Myc tag was immunoprecipitated in 2µg N terminal Myc Tag fusion protein lysate with EM31105 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using R1208-1 at 1/1,000 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature. Lane 1: Myc Tag fusion protein lysate (input). Lane 2: EM1902-38 IP in Myc Tag fusion protein lysate. Lane 3: EM31105 IP in Myc Tag fusion protein lysate. Lane 4: Mouse IgG instead of EM31105 in Myc Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.