EM40307

Product Type:	Mouse monoclonal IgG2a, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	9-B9

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 41 kDa

Isotype:

IgG2a

Immunogen:

Synthetic peptide within Human MAPK14 aa 1-50 / 360.

Positive control:

HepG2 cell lysates, A431 cell lysates, NIH/3T3 cell lysates, Hela cell lysates, Jurkat cell lysates, 293T cell lysates, mouse placenta tissue lysates, Hela, human colon carcinoma tissue,human stomach carcinoma tissue, mouse stomach tissue.

Subcellular location:

Cytoplasm, nucleus

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:100-1:200
1:100-1:200

Uniprot #:

SwissProt: Q16539 Human | P47811 Mouse

Alternative names:

CSAID Binding Protein 1 CSAID binding protein CSAID-binding protein Csaids binding protein CSBP 1 CSBP 2 CSBP CSBP1 CSBP2 CSPB1 Cytokine suppressive anti-inflammatory drug-binding protein EXIP MAP kinase 14 MAP kinase MXI2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX interacting protein 2 MAX-interacting protein 2 Mitogen Activated Protein Kinase 14 Mitogen activated protein kinase p38 alpha Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 alpha MK14_HUMAN Mxi 2 MXI2 p38 ALPHA p38 p38 MAP kinase p38 MAPK p38 mitogen activated protein kinase p38ALPHA p38alpha Exip PRKM14 PRKM15 RK SAPK2A

	Fig1: Western blot analysis of MAPK14 on different lysates using anti-MAPK14 antibody at 1/500 dilution. Positive control: Lane 1: A431 cell lysates Lane 2: HepG2 cell lysates Lane 3: Hela cell lysates Lane 4: NIH/3T3 cell lysates Lane 5: Jurkat cell lysates Lane 6: 293T cell lysates Lane 7: mouse placenta tissue lysates
	Fig2: ICC staining of MAPK14 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.