|Product Type:||Mouse monoclonal IgG1, primary antibodies|
|Species reactivity:||Human, Mouse|
|Applications:||WB, IHC-P, FC|
|Storage condition:||Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.|
|Storage buffer:||1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.|
|Purification:||Immunogen affinity purified.|
|Molecular weight:||Predicted band size: 61 kDa|
|Immunogen:||Synthetic peptide within human CD19 aa 110-190.|
|Positive control:||Raji cell lysate, Daudi cell lysate, mouse lymphoid lysates, human spleen tissue, Daudi.|
|Uniprot #:||SwissProt: P15391 Human | P25918 Mouse
|Alternative names:||deficiency due to defect in CD19, included AW495831 B lymphocyte antigen CD19 B lymphocyte surface antigen B4 B-lymphocyte antigen CD19 B-lymphocyte surface antigen B4 B4 CD19 CD19 antigen CD19 molecule Cd19 protein CD19_HUMAN CVID3 Differentiation antigen CD19 Leu 12 Leu-12 Leu12 MGC109570 MGC12802 T-cell surface antigen Leu-12|
Western blot analysis of CD19 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM40308, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Western blot analysis of CD19 on mouse lymphoid lysates with Mouse anti-CD19 antibody (EM40308) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40308) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
|Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM40308, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.|
|Fig4: Flow cytometric analysis of CD19 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (EM40308, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).|