AKT1 Mouse Monoclonal Antibody [D9-9-C9]
cat.: EM40507
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | D9-9-C9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within human AKT1 aa 1-80. |
| Positive control: | MCF7 cell lysate, A549 cell lysate, Jurkat cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, Hela, HepG2, MCF-7, human breast carcinoma tissue. |
| Subcellular location: | Nucleus, cytoplasm, cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000 1:100-1:200 1:100-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P31749 Human | P31750 Mouse | P47196 Rat |
| Alternative names: | AKT 1 AKT AKT1 AKT1_HUMAN MGC99656 PKB PKB-ALPHA PRKBA Protein Kinase B Alpha Protein kinase B Proto-oncogene c-Akt RAC Alpha RAC RAC-alpha serine/threonine-protein kinase RAC-PK-alpha |
Images
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Fig1:
Western blot analysis of AKT1 on different lysates with Mouse anti-AKT1 antibody (EM40507) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: A549 cell lysate Lane 3: Jurkat cell lysate Lane 4: C2C12 cell lysate Lane 5: L-929 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40507) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Akt1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Akt1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Akt1 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Akt1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with Akt1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.