IDH1 Mouse Monoclonal Antibody [A11-B4]
cat.: EM40705
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A11-B4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human IDH1 aa 101-150 / 414. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human breast tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: O75874 Human | O88844 Mouse | P41562 Rat |
| Alternative names: | Cytosolic NADP isocitrate dehydrogenase Cytosolic NADP-isocitrate dehydrogenase Epididymis luminal protein 216 Epididymis secretory protein Li 26 HEL-216 HEL-S-26 ICDH IDCD IDH IDH1 IDHC_HUMAN IDP IDPC Isocitrate dehydrogenase [NADP] cytoplasmic Isocitrate dehydrogenase 1 (NADP+) soluble NADP dependent isocitrate dehydrogenase cytosolic NADP dependent isocitrate dehydrogenase peroxisomal NADP(+)-specific ICDH Oxalosuccinate decarboxylase PICD |
Images
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Fig1:
Western blot analysis of IDH1 on different lysates with Mouse anti-IDH1 antibody (EM40705) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: A431 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: PC-12 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 42 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40705) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IDH1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-IDH1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IDH1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.