Glutathione Peroxidase 1 Mouse Monoclonal Antibody [C5-F6]
cat.: EM40708
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | C5-F6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human Glutathione Peroxidase 1 aa 25-203 / 203. |
| Positive control: | THP-1 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, HT-29 cell lysate, Hela, HepG2, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000 1:200 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P07203 Human |
| Alternative names: | AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245 |
Images
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Fig1:
Western blot analysis of Glutathione Peroxidase 1 on different lysates with Mouse anti-Glutathione Peroxidase 1 antibody (EM40708) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40708) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.