Product Type: | Mouse monoclonal IgG2b, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | C5-F6 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 22 kDa |
Isotype: | IgG2b |
Immunogen: | Recombinant protein within Human Glutathione Peroxidase 1 aa 25-203 / 203. |
Positive control: | Hela, HepG2, human kidney tissue, THP-1 cell lysates, HepG2 cell lysates, 293T cell lysates. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:200 1:200 1:100-1:200 |
Uniprot #: | SwissProt: P07203 Human |
Alternative names: | AL033363 Cellular glutathione peroxidase Glutathione peroxidase 1 Glutathione peroxidase GPx 1 GPx-1 GPX1 GPX1_HUMAN GPXD GSHPx-1 GSHPX1 MGC14399 MGC88245 |
Fig1:
Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1,000 dilution. Positive control: Lane 1: THP-1 cell lysates Lane 2: HepG2 cell lysates Lane 3: 293T cell lysates |
|
Fig2: ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining GPX1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of HepG2 cells with GPX1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |