Caveolin-1 Mouse Monoclonal Antibody [19-D6]
cat.: EM40728
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 19-D6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Mouse Caveolin-1 aa 129-178 / 178. |
| Positive control: | A431 cell lysates, NIH/3T3 cell lysates, L929, rat lung tissue, human lung tissue, mouse lung tissue, mouse liver tissue, mouse bladder tissue. |
| Subcellular location: | Cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:500 1:200 1:200 |
| Uniprot #: | SwissProt: Q03135 Human | P49817 Mouse | P41350 Rat |
| Alternative names: | BSCL3 CAV CAV1 CAV1_HUMAN caveolae protein, 22 kD caveolin 1 alpha isoform caveolin 1 beta isoform Caveolin 1 caveolae protein 22kDa Caveolin-1 Caveolin1 cell growth-inhibiting protein 32 CGL3 LCCNS MSTP085 OTTHUMP00000025031 PPH3 VIP 21 VIP21 |
Images
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Fig1:
Western blot analysis of Caveolin-1 on A431 cell lysates with Mouse anti-Caveolin-1 antibody (EM40728) at different dilutions. Lane 1: 1/200 dilution Lane 2: 1/500 dilution Lane 3: 1/1,000 dilution Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Caveolin-1 on NIH/3T3 cell lysates with Mouse anti-Caveolin-1 antibody (EM40728) at different dilutions. Lane 1: 1/200 dilution Lane 2: 1/500 dilution Lane 3: 1/1,000 dilution Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 23 kDa Exposure time: 1 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40728) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining Caveolin-1 in L929 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Caveolin-1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.