FGFR2/CD332 Recombinant Mouse Monoclonal Antibody [A5-E10-G7-R]
cat.: EM50103
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A5-E10-G7-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human FGFR2 / CD332 aa 601-723 / 821. |
| Positive control: | MCF7 cell lysate, K-562 cell lysate, HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, Jurkat cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Golgi apparatus, Membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P21802 Human |
| Alternative names: | bacteria-expressed kinase BBDS BEK BEK fibroblast growth factor receptor BFR1 CD332 CD332 antigen CEK3 CFD1 Craniofacial dysostosis 1 ECT1 FGF receptor FGFR 2 FGFR-2 Fgfr2 FGFR2_HUMAN Fibroblast growth factor receptor 2 Hydroxyaryl protein kinase Jackson Weiss syndrome JWS K SAM K-sam Keratinocyte growth factor receptor 2 Keratinocyte growth factor receptor KGFR KSAM protein tyrosine kinase, receptor like 14 soluble FGFR4 variant 4 TK14 TK25 |
Images
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Fig1:
Western blot analysis of FGFR2/CD332 on different lysates with Mouse anti-FGFR2/CD332 antibody (EM50103) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: A431 cell lysate Lane 6: A549 cell lysate Lane 7: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 140 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50103) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.