Ku70 Mouse Monoclonal Antibody [D1-A6]
cat.: EM50109
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: D1-A6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 70 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within human Ku70 aa 1-150/609.
Positive control: A549 cell lysate, HeLa cell lysate, COS-1 cell lysate, human breast cancer tissue, human tonsils tissue, mouse colon tissue, mouse kidney tissue, mouse spleen tissue, rat kidney tissue.
Subcellular location: Nucleus, Chromosome.
Recommended Dilutions:
  WB
  IHC-P
1:2,000
1:1,000-1:2,000
Uniprot #: SwissProt: P12956 Human | P23475 mouse
Entrez Gene: 25019 Rat
Alternative names: 5'-deoxyribose-5-phosphate lyase Ku70 5'-dRP lyase Ku70 70 kDa subunit of Ku antigen ATP dependent DNA helicase 2 subunit 1 ATP dependent DNA helicase II 70 kDa subunit ATP-dependent DNA helicase 2 subunit 1 ATP-dependent DNA helicase II 70 kDa subunit CTC box binding factor 75 kDa subunit CTC box-binding factor 75 kDa subunit CTC75 CTCBF DNA repair protein XRCC6 G22P1 Ku 70 Ku autoantigen p70 subunit Ku autoantigen, 70kDa Ku p70 Ku70 Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa Kup70 Lupus Ku autoantigen protein p70 ML8 Thyroid autoantigen 70kD (Ku antigen) Thyroid autoantigen Thyroid lupus autoantigen Thyroid lupus autoantigen p70 Thyroid-lupus autoantigen TLAA X ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair cross-complementing protein 6 XRCC 6 Xrcc6 XRCC6_HUMAN
Images
EM50109_1.jpg Fig1: Western blot analysis of Ku70 on different lysates with Mouse anti-Ku70 antibody (EM50109) at 1/2,000 dilution.

Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: COS-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM50109) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM50109_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Ku70 antibody (EM50109) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM50109_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Mouse anti-Ku70 antibody (EM50109) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM50109_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-Ku70 antibody (EM50109) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM50109_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Ku70 antibody (EM50109) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM50109_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-Ku70 antibody (EM50109) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM50109_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-Ku70 antibody (EM50109) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM50109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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