EM50109

Product Type:	Mouse monoclonal IgG2a, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P
Clonality:	Monoclonal
Clone number:	D1-A6

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 70 kDa

Isotype:

IgG2a

Immunogen:

Recombinant protein within human Ku70 aa 1-150/609.

Positive control:

Hela cell lysates, HepG2 cell lysates, A431 cell lysates, D3 cell lysates, 293T cell lysates, A549 cell lysates, COS-1 cell lysates, human kidney tissue lysates, human liver tissue lysates, human brain tissue lysates, human thymus tissue lysates, human placentae tissue lysates, mouse kidney tissue lysates, mouse liver tissue lysates, mouse thymus tissue lysates, mouse testis tissue lysates, human tonsil tissue,human breast cancer tissue.

Subcellular location:

Nucleus.

Recommended Dilutions:
WB
IHC-P

1:2,000
1:100-1:200

Uniprot #:

SwissProt: P12956 Human | P23475 mouse

Alternative names:

5'-deoxyribose-5-phosphate lyase Ku70 5'-dRP lyase Ku70 70 kDa subunit of Ku antigen ATP dependent DNA helicase 2 subunit 1 ATP dependent DNA helicase II 70 kDa subunit ATP-dependent DNA helicase 2 subunit 1 ATP-dependent DNA helicase II 70 kDa subunit CTC box binding factor 75 kDa subunit CTC box-binding factor 75 kDa subunit CTC75 CTCBF DNA repair protein XRCC6 G22P1 Ku 70 Ku autoantigen p70 subunit Ku autoantigen, 70kDa Ku p70 Ku70 Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa Kup70 Lupus Ku autoantigen protein p70 ML8 Thyroid autoantigen 70kD (Ku antigen) Thyroid autoantigen Thyroid lupus autoantigen Thyroid lupus autoantigen p70 Thyroid-lupus autoantigen TLAA X ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair complementing defective repair in Chinese hamster cells 6 X-ray repair cross-complementing protein 6 XRCC 6 Xrcc6 XRCC6_HUMAN

	Fig1: Western blot analysis of Ku70 on different cell lysates using anti-Ku70 antibody at 1/2000 dilution. Positive control: Lane 1: Hela cell lysates Lane 2: HepG2 cell lysates Lane 3: A431 cell lysates Lane 4: D3 cell lysates Lane 5: 293T cell lysates Lane 6: A549 cell lysates Lane 7: COS-1 cell lysates Lane 8: Human kidney tissue lysates Lane 9: Human liver tissue lysates Lane 10: human brain tissue lysates Lane 11: Human thymus tissue lysates Lane 12: Human placentae tissue lysates Lane 13: Mouse kidney tissue lysates Lane 14: Mouse liver tissue lysates Lane 15: Mouse thymus tissue lysates Lane 16: Mouse testis tissue lysates
	Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku70 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ku70 antibody. Counter stained with hematoxylin.