CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6]
cat.: EM50804
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Staphylococcus aureus |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 8-A6-6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within Streptococcus Pyogenes CRISPR-Cas9 aa1-200. |
| Positive control: | Human 293T cells transfected with CRISPR-Cas9 lysate. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q99ZW2 Streptococcus Pyogenes |
| Alternative names: | Cas9 CRISPR-associated endonuclease Cas9/Csn1 CRISPR-Cas9/Csn1 csn1 SpyCas9 |
Images
|
Fig1:
Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5,000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate |
|
Fig2: Immunofluorescence staining CRISPR-Cas9 in CRISPR-Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3:
Immunocytochemistry analysis of 293T cells transfected with or without px459 labeling CRISPR-Cas9 with Mouse anti-CRISPR-Cas9 antibody (EM50804) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CRISPR-Cas9 antibody (EM50804) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.