HSP70 Mouse Monoclonal Antibody
cat.: EM60062
| Product Type: | Mouse monoclonal, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 70 kDa |
| Immunogen: | Synthetic Peptide of HSP70 |
| Positive control: | Stomach mucosa,Testis, |
| Subcellular location: | Cytosol, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-cell IHC-P |
1:1000-1:2000 1:100-200 1:50-300 |
| Uniprot #: | SwissProt: P0DMV8 Human | P0DMV9 Human |
| Alternative names: | DnaK type molecular chaperone HSP70 1 Epididymis secretory protein Li 103 Heat shock 70 kDa protein 1 Heat shock 70 kDa protein 1/2 Heat shock 70 kDa protein 1A Heat shock 70 kDa protein 1A/1B Heat shock 70kDa protein 1B Heat shock induced protein HSP70 1 HSP70 1A HSP70 1B HSP70 2 HSP70-1 HSP70-1/HSP70-2 HSP70-1A HSP70-2 HSP70.1 HSP70.1/HSP70.2 HSP71_HUMAN HSP72 HSPA1 HSPA1A HSPA1B |
Images
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Fig1: Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody (cat: HA1006)was diluted at 1:10000, 37° 1hour. |
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Fig2: Immunofluorescence analysis of Human-breast-cancer tissue. 1, HSP70 Monoclonal Antibody (red) was diluted at 1:200 (4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B. |
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Fig3: Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, HSP70 Monoclonal Antibody was diluted at 1:200 (4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98℃,20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.