GST Mouse Monoclonal Antibody [16-21]
cat.: EM80701
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 16-21 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein. |
| Positive control: | GST-tagged recombinant protein. |
| Recommended Dilutions:
WB ELISA |
1:5,000 1:10,000-1:20,000 |
| Alternative names: | Glutathione S Transferase Glutathione S-transferase class-mu 26 kDa isozyme GST Sj26 antigen SjGST |
Images
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Fig1:
Western blot analysis of GST on different lysates with Mouse anti-GST antibody (EM80701) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM80701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.