Insulin Mouse Monoclonal Antibody [A6-6]
cat.: EM80714
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, ELISA, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | A6-6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Insulin aa 25-54. |
| Positive control: | Human pancreas tissue, mouse pancreas tissue, rat pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P ELISA IF-Tissue IHC-Fr |
1:2,000-1:5,000 1:10,000 1:200-1:1,000 1:500 |
| Uniprot #: | SwissProt: P01308 Human | P01325 Mouse | P01322 Rat |
| Alternative names: | IDDM IDDM1 IDDM2 ILPR ins INS_HUMAN Insulin A chain Insulin B chain IRDN MODY10 Preproinsulin Proinsulin Proinsulin precursor |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Pancreas Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: Insulin (EM80714, 1/500, Red), Glucagon (ET1702-20, 1/500, green), overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Pancreas Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: Insulin (EM80714, 1/500, Red), PPY (HA721515, 1/500, green), overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Pancreas Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: Insulin (EM80714, 1/500, Red), PPY (HA721515, 1/500, green), overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Insulin antibody (EM80714) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM80714) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig7:
Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Insulin with Mouse anti-Insulin antibody (EM80714) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM80714, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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