LMNB2 Rabbit Polyclonal Antibody
cat.: ER0403
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to of Human LMNB2 aa 571-620 / 620. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HeLa, mouse embryonic stem cells, human colon cancer tissue. |
| Subcellular location: | Nucleus inner membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:250 1:200 |
| Uniprot #: | SwissProt: Q03252 Human | P21619 Mouse Entrez Gene: 299625 Rat |
| Alternative names: | LAMB 2 LAMB2 Lamin-B2 Lamin B2 LMN 2 LMN B2 LMN2 LMNB 2 LMNB2 LMNB2_HUMAN MGC2721 RGD1563803 |
Images
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Fig1:
Western blot analysis of LMNB2 on different lysates with Rabbit anti-LMNB2 antibody (ER0403) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0403) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling LMNB2 with Rabbit anti-LMNB2 antibody (ER0403) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LMNB2 antibody (ER0403) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: ICC staining of LMNB2 in mouse embryonic stem cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-LMNB2 antibody (ER0403) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER0403) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.