Anti-N-Cadherin antibody
cat.: ER0503
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified
Molecular weight: 140 kDa
Isotype: IgG
Immunogen: Synthetic peptide (KLH-coupled) within human N-cadherin 360-420 aa.
Positive control: Mouse heart, human heart, 293, mouse embryonic stem cell, HepG2 Human colon?carcinoma tissue, human liver?carcinoma tissue
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:200
1:200
1:50
Uniprot #: SwissProt: P19022 Human | P15116 Mouse
Alternative names: CADH2_HUMAN antibody Cadherin 2 antibody Cadherin 2 N cadherin neuronal antibody Cadherin 2 type 1 antibody Cadherin 2 type 1 N cadherin neuronal antibody Cadherin 2, type 1, N-cadherin (neuronal) antibody Cadherin-2 antibody Cadherin2 antibody Calcium dependent adhesion protein neuronal antibody CD325 antibody CD325 antigen antibody CDH2 antibody CDHN antibody CDw325 antibody CDw325 antigen antibody N cadherin 1 antibody N-cadherin antibody NCAD antibody Neural cadherin antibody OTTHUMP00000066304 antibody OTTHUMP00000067378 antibody
Images
ER0503_1.jpg Fig1: Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart lysates
Lane2 : Human heart lysates
ER0503_2.jpg Fig2: ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0503_3.jpg Fig3: ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0503_4.jpg Fig4: ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0503_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
ER0503_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.