N-Cadherin Rabbit Polyclonal Antibody
cat.: ER0503
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | -15℃ to -25℃/1 year(Do not lower than -25℃) |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human N-Cadherin aa 391-440 / 906 (H412G). |
| Positive control: | Brain,Epithelium,Liver |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IHC IF ELISA |
1:500-1:2,000 1:100-1:300 1:200-1:1,000 1:10,000 |
| Uniprot #: | SwissProt: P19022 Human | P15116 Mouse | Q9Z1Y3 Rat |
| Alternative names: | CADH2_HUMAN Cadherin 2 Cadherin 2 N cadherin neuronal Cadherin 2 type 1 Cadherin 2 type 1 N cadherin neuronal Cadherin 2, type 1, N-cadherin (neuronal) Cadherin-2 Cadherin2 Calcium dependent adhesion protein neuronal CD325 CD325 antigen CDH2 CDHN CDw325 CDw325 antigen N cadherin 1 N-cadherin NCAD Neural cadherin OTTHUMP00000066304 OTTHUMP00000067378 |
Images
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Fig1: Immunofluorescence analysis of rat-lung tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Fig2: Immunofluorescence analysis of rat-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Fig3: Immunofluorescence analysis of mouse-kidney tissue. 1,N-Cadherin Polyclonal Antibody(red) was diluted at 1:200(4℃,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B |
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Fig4: Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4℃,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98℃,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
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Fig5: Immunohistochemical analysis of paraffin-embedded Human kidney. 1,N-Cadherin Polyclonal Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
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Fig6: Western Blot analysis of Jurkat cells using N-Cadherin Polyclonal Antibody diluted at 1:1000 |
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Fig7: Western Blot analysis of K562 cells using N-Cadherin Polyclonal Antibody diluted at 1:1000 |
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Fig8: Western blot analysis of lysates from COLO205 and K562 cells, using CDH2 Antibody. The lane on the right is blocked with the synthesized peptide. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.