N-Cadherin Rabbit Polyclonal Antibody
cat.: ER0503
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 140 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human N-Cadherin aa 391-440 / 906 (H412G). |
| Positive control: | Mouse heart, human heart, 293, mouse embryonic stem cell, HepG2 Human colon?carcinoma tissue, human liver?carcinoma tissue |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:200 1:200 1:50 |
| Uniprot #: | SwissProt: P19022 Human | P15116 Mouse |
| Alternative names: | CADH2_HUMAN Cadherin 2 Cadherin 2 N cadherin neuronal Cadherin 2 type 1 Cadherin 2 type 1 N cadherin neuronal Cadherin 2, type 1, N-cadherin (neuronal) Cadherin-2 Cadherin2 Calcium dependent adhesion protein neuronal CD325 CD325 antigen CDH2 CDHN CDw325 CDw325 antigen N cadherin 1 N-cadherin NCAD Neural cadherin OTTHUMP00000066304 OTTHUMP00000067378 |
Images
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Fig1:
Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart lysates Lane2 : Human heart lysates |
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Fig2: ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.