Bcl-2 Rabbit Polyclonal Antibody
cat.: ER0602
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 26 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Bcl-2.
Positive control: Jurkat cell lysate, HL-60 cell lysate, THP-1 cell lysate, Hela, human colon carcinoma tissue, human tonsil tissue.
Subcellular location: Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:5,000
1:200
1:200
1:50-1:100
Uniprot #: SwissProt: P10415 Human
Alternative names: Apoptosis regulator Bcl 2 Apoptosis regulator Bcl-2 Apoptosis regulator Bcl2 AW986256 B cell CLL/lymphoma 2 B cell leukemia/lymphoma 2 Bcl-2 Bcl2 BCL2_HUMAN C430015F12Rik D630044D05Rik D830018M01Rik Leukemia/lymphoma, B-cell, 2 Oncogene B-cell leukemia 2 PPP1R50 Protein phosphatase 1, regulatory subunit 50 Bcl 2
Images
ER0602_1.jpg Fig1: Western blot analysis of Bcl-2 on different lysates with Rabbit anti-Bcl-2 antibody (ER0602) at 1/5,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: HL-60 cell lysate
Lane 3: THP-1 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 26 kDa
Observed band size: 26 kDa

Exposure time: 1 minute 2 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0602) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
ER0602_2.jpg Fig2: ICC staining Bcl-2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0602_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
ER0602_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
ER0602_5.jpg Fig5: Flow cytometric analysis of Hela cells with Bcl-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.