Cyclin D1 Rabbit Polyclonal Antibody
cat.: ER0722
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse Cyclin D1 aa 1-50 / 295. |
| Positive control: | Mouse brain tissue lysates, human tonsil tissue, human liver carcinoma tissue, A431. |
| Subcellular location: | Nucleus, Cytoplasm, Nucleus membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000-1:5,000 1:200 1ug/mL |
| Uniprot #: | SwissProt: P24385 Human | P25322 Mouse | P39948 Rat |
| Alternative names: | AI327039 B cell CLL/lymphoma 1 B cell leukemia 1 B cell lymphoma 1 protein B-cell lymphoma 1 protein BCL 1 BCL-1 BCL-1 oncogene BCL1 BCL1 oncogene ccnd1 CCND1/FSTL3 fusion gene, included CCND1/IGHG1 fusion gene, included CCND1/IGLC1 fusion gene, included CCND1/PTH fusion gene, included CCND1_HUMAN cD1 Cyl 1 D11S287E G1/S specific cyclin D1 G1/S-specific cyclin-D1 Parathyroid adenomatosis 1 PRAD1 PRAD1 oncogene U21B31 |
Images
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Fig1:
Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution. Positive control: mouse brain |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin. |
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Fig4:
Flow cytometric analysis of A431 cells labeling Cyclin D1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER0722, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.