Cyclin D1 Rabbit Polyclonal Antibody
cat.: ER0722
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 34 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Mouse Cyclin D1 aa 1-50 / 295.
Positive control: Mouse brain tissue lysates, human tonsil tissue, human liver carcinoma tissue, A431.
Subcellular location: Nucleus, Cytoplasm, Nucleus membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:2,000-1:5,000
1:200
1ug/mL
Uniprot #: SwissProt: P24385 Human | P25322 Mouse | P39948 Rat
Alternative names: AI327039 B cell CLL/lymphoma 1 B cell leukemia 1 B cell lymphoma 1 protein B-cell lymphoma 1 protein BCL 1 BCL-1 BCL-1 oncogene BCL1 BCL1 oncogene ccnd1 cyclind1 CCND1/FSTL3 fusion gene, included CCND1/IGHG1 fusion gene, included CCND1/IGLC1 fusion gene, included CCND1/PTH fusion gene, included CCND1_HUMAN cD1 Cyl 1 D11S287E G1/S specific cyclin D1 G1/S-specific cyclin-D1 Parathyroid adenomatosis 1 PRAD1 PRAD1 oncogene U21B31
Images
ER0722_1.jpg Fig1: Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution.
Positive control: mouse brain
ER0722_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
ER0722_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
ER0722_4.jpg Fig4: Flow cytometric analysis of A431 cells labeling Cyclin D1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER0722, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.