BAX Rabbit Polyclonal Antibody
cat.: ER0907
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 21 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human BAX aa 1-50 / 192.
Positive control: HepG2, Hela, mouse embryonic stem cell, PC12, F9 293, human gall bladder tissue, human cervical tissue, mouse testis tissue
Subcellular location: Mitochondrion membrane, cytoplasm
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:1,000
1:200
1:200
Uniprot #: SwissProt: Q07812 Human | Q07813 Mouse | Q63690 Rat
Alternative names: Apoptosis regulator BAX BAX Bax-protein BAX_HUMAN BAXA Baxdelta2G9 Baxdelta2G9omega Baxdelta2omega Bcl-2-like protein 4 BCL2 associated X protein BCL2 associated X protein omega BCL2 associated X protein transcript variant delta2 Bcl2-L-4 BCL2L4 membrane isoform alpha
Images
ER0907_1.jpg Fig1: Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2 Lane2 :Hela Lane 3:Mouse embryonic stem cell Lane 4:PC12 Lane 5:F9
ER0907_2.jpg Fig2: ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0907_3.jpg Fig3: ICC staining Bax in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0907_4.jpg Fig4: ICC staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0907_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.
ER0907_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.
ER0907_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.