HMGB1 Rabbit Polyclonal Antibody
cat.: ER0913
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HMGB1 aa 71-126. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Hela, NIH/3T3, mouse kidney tissue, mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue, |
| Subcellular location: | Nucleus, Cell membrane, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P09429 Human |
| Alternative names: | Amphoterin Chromosomal protein, nonhistone, HMG1 DKFZp686A04236 High mobility group 1 High mobility group box 1 High mobility group protein 1 High mobility group protein B1 high-mobility group (nonhistone chromosomal) protein 1 HMG-1 HMG1 HMG3 HMGB 1 HMGB1 HMGB1_HUMAN NONHISTONE CHROMOSOMAL PROTEIN HMG1 SBP 1 Sulfoglucuronyl carbohydrate binding protein |
Images
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Fig1:
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (ER0913) at 1/2,000 dilution. Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate (15 µg/Lane) Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate (15 µg/Lane) Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate (15 µg/Lane) Lane 5: Mouse kidney tissue lysate (30 µg/Lane) Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER0913, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 25 kDa |
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Fig2: ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining HMGB1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.