HMGB1 Rabbit Polyclonal Antibody
cat.: ER0913
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human HMGB1 aa 71-126. |
| Positive control: | Wild-type Raw264.7 whole cell lysate, HepG2 cell lysate, MCF-7 cell lysate, F9 cell lysate, A549 cell lysate, Hela, NIH/3T3, mouse kidney tissue, mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue, |
| Subcellular location: | Nucleus, Cell membrane, Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:200 1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P09429 Human |
| Alternative names: | Amphoterin Chromosomal protein, nonhistone, HMG1 DKFZp686A04236 High mobility group 1 High mobility group box 1 High mobility group protein 1 High mobility group protein B1 high-mobility group (nonhistone chromosomal) protein 1 HMG-1 HMG1 HMG3 HMGB 1 HMGB1 HMGB1_HUMAN NONHISTONE CHROMOSOMAL PROTEIN HMG1 SBP 1 Sulfoglucuronyl carbohydrate binding protein |
Images
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Fig1:
All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (ER0913) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockdown Raw264.7 whole cell lysate. ER0913 was shown to specifically react with HMGB1 in Wild-type Raw264.7 cells. Weakened band was observed when HMGB1 knockdown sample was tested. Wild-type and HMGB1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (ER0913, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2:
Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 cell lysate Lane 2: MCF-7 cell lysate Lane 3: F9 cell lysate Lane 4: A549 cell lysate |
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Fig3: ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining HMGB1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- HMGB1 antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of Hela cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.