HMGB1 Rabbit Polyclonal Antibody
cat.: ER0913
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human HMGB1 aa 71-126.
Positive control: HepG2, MCF-7, F9, A549, PC12, Hela, NIH/3T3, RAW264.7, mouse kidney tissue Mouse stomach tissue, mouse brain tissue, human stomach carcinoma tissue, human tonsil tissue
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:200
1:200
1:50-1:100
Uniprot #: SwissProt: P09429 Human
Alternative names: Amphoterin Chromosomal protein, nonhistone, HMG1 DKFZp686A04236 High mobility group 1 High mobility group box 1 High mobility group protein 1 High mobility group protein B1 high-mobility group (nonhistone chromosomal) protein 1 HMG-1 HMG1 HMG3 HMGB 1 HMGB1 HMGB1_HUMAN NONHISTONE CHROMOSOMAL PROTEIN HMG1 SBP 1 Sulfoglucuronyl carbohydrate binding protein
Images
ER0913_1.jpg Fig1: All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (ER0913) at 1:500 dilution.
Lane 1: Wild-type Raw264.7 whole cell lysate.
Lane 2: HMGB1 knockdown Raw264.7 whole cell lysate.

ER0913 was shown to specifically react with HMGB1 in Wild-type Raw264.7 cells. Weakened band was observed when HMGB1 knockdown samples were tested. Wild-type and HMGB1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (ER0913, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER0913_2.jpg Fig2: Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: F9
Lane 4: A549
ER0913_3.jpg Fig3: ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0913_4.jpg Fig4: ICC staining HMGB1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER0913_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
ER0913_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
ER0913_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
ER0913_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
ER0913_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
ER0913_10.jpg Fig10: Flow cytometric analysis of HMGB1 cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.