LGR5 Rabbit Polyclonal Antibody
cat.: ER10708
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Mouse GPR49 / LGR5 alpha / beta aa 1-50 / 907 (ID: Q9Z1P4). |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, F9 cell lysate, Hela cell lysate, K562 cell lysate. |
| Subcellular location: | Cytoplasm, cell membrane, Golgi apparatus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O75473 Human | Q9Z1P4 Mouse Entrez Gene: 299802 Rat |
| Alternative names: | FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38 |
Images
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Fig1:
Western blot analysis of LGR5 on different lysates with Rabbit anti-LGR5 antibody (ER10708) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER10708) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of LGR5 on different cell lysates using anti- GPR49/LGR5 alpha/beta antibody at 1/1,000 dilution. Positive control: Lane 1: F9 cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.