BDNF Rabbit Polyclonal Antibody
cat.: ER130915
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human BDNF.
Positive control: A172 cell lysates, SHG-44 cell lysates, mouse heart tissue lysates, mouse brain tissue lysates, SHG-44, human lung tissue, mouse lung tissue, mouse testis tissue, mouse heart tissue, rat spinal cord tissue lysates
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:200
1:200
Uniprot #: SwissProt: P23560 Human | P21237 Mouse | P23363 Rat
Alternative names: Abrineurin ANON2 BDNF BDNF_HUMAN Brain Derived Neurotrophic Factor Brain-derived neurotrophic factor BULN2 MGC34632 Neurotrophin
Images
ER130915_1.jpg Fig1: Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution.
Positive control:
Lane 1: A172 cell lysates
Lane 2: SHG-44 cell lysates
Lane 3: Mouse heart tissue lysates
Lane 4: Mouse brain tissue lysates
ER130915_2.jpg Fig2: Western blot analysis of BDNF on rat spinal cord tissue lysates with Rabbit anti-BDNF antibody (ER130915) at 1/500 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER130915) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER130915_3.jpg Fig3: ICC staining of BNDF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER130915_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER130915_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER130915_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER130915_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-BDNF antibody (ER130915) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER130915) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.