Anti-ERK1 antibody
cat.: ER131011
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 44 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ERK1 aa 306-360.
Positive control: Hela, SW480, HCT116, HepG2, PC12, LOVO, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue
Subcellular location: Cytoplasm, nucleus
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:1,000
1:200
1:200
Uniprot #: SwissProt: P27361 Human
Alternative names: ERK 1 ERK ERK-1 ERK1 ERT 2 ERT2 Extracellular Signal Regulated Kinase 1 Extracellular signal related kinase 1 Extracellular signal-regulated kinase 1 HGNC6877 HS44KDAP HUMKER1A Insulin Stimulated MAP2 Kinase Insulin-stimulated MAP2 kinase MAP kinase 1 MAP kinase 3 MAP Kinase MAP kinase isoform p44 MAPK 1 MAPK 3 MAPK MAPK1 Mapk3 MGC20180 Microtubule Associated Protein 2 Kinase Microtubule-associated protein 2 kinase Mitogen Activated Protein Kinase 3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 3 MK03_HUMAN OTTHUMP00000174538 OTTHUMP00000174541 p44 ERK1 p44 MAPK p44-ERK1 p44-MAPK P44ERK1 P44MAPK PRKM 3 PRKM3 Protein Kinase Mitogen Activated 3
Images
ER131011_1.jpg Fig1: Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: SW480
Lane 3: HCT116
Lane 4: HepG2
Lane 5: PC12
ER131011_2.jpg Fig2: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER131011_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
ER131011_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
ER131011_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.