ERK1/2 Rabbit Polyclonal Antibody
cat.: ER131011
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 43/41 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ERK1 aa 306-360.
Positive control: HeLa cell lysate, Jurkat cell lysate, Ramos cell lysate, MCF7 cell lysate, Neuro-2a cell lysate, C6 cell lysate, HeLa, RAW264.7, PC-12, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue.
Subcellular location: Cytoplasm, Nucleus, Cell junction, Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:2,000-1:5,000
1:200
1:200
Uniprot #: SwissProt: P27361 Human | P28482 Human | P63085 Mouse | Q63844 Mouse | P21708 Rat | P63086 Rat
Alternative names: ERK 1 ERK 2 ERK-2 ERK1 erk1/2 ERK2 ERT1 ERT2 Extracellular signal regulated kinase 1 Extracellular signal-regulated kinase 2 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAP kinase isoform p44 MAPK 1 MAPK 2 MAPK 3 Mapk1 MAPK2 MAPK3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 MK01_HUMAN p38 p40 p41 p42-MAPK PRKM 2
Images
ER131011_1.jpg Fig1: Western blot analysis of ERK1/2 on different lysates with Rabbit anti-ERK1/2 antibody (ER131011) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Ramos cell lysate
Lane 4: MCF7 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43/41 kDa
Observed band size: 43/41 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER131011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER131011_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER131011_3.jpg Fig3: Immunocytochemistry analysis of RAW264.7 cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER131011_4.jpg Fig4: Immunocytochemistry analysis of PC-12 cells labeling ERK1/2 with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1/2 antibody (ER131011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER131011_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
ER131011_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
ER131011_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1/2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.