ERK1 Rabbit Polyclonal Antibody
cat.: ER131011
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ERK1 aa 306-360. |
| Positive control: | Hela cell lysate, SW480 cell lysate, HCT116 cell lysate, HepG2 cell lysate, PC12 cell lysate, F9 cell lysate, LOVO, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cell junction, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:5,000 1:200 1:200 |
| Uniprot #: | SwissProt: P27361 Human |
| Alternative names: | ERK 1 ERK ERK-1 ERK1 ERT 2 ERT2 Extracellular Signal Regulated Kinase 1 Extracellular signal related kinase 1 Extracellular signal-regulated kinase 1 HGNC6877 HS44KDAP HUMKER1A Insulin Stimulated MAP2 Kinase Insulin-stimulated MAP2 kinase MAP kinase 1 MAP kinase 3 MAP Kinase MAP kinase isoform p44 MAPK 1 MAPK 3 MAPK MAPK1 Mapk3 MGC20180 Microtubule Associated Protein 2 Kinase Microtubule-associated protein 2 kinase Mitogen Activated Protein Kinase 3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 3 MK03_HUMAN OTTHUMP00000174538 OTTHUMP00000174541 p44 ERK1 p44 MAPK p44-ERK1 p44-MAPK P44ERK1 P44MAPK PRKM 3 PRKM3 Protein Kinase Mitogen Activated 3 |
Images
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Fig1:
Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: SW480 cell lysate Lane 3: HCT116 cell lysate Lane 4: HepG2 cell lysate Lane 5: PC12 cell lysate |
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Fig2:
Western blot analysis of ERK1 on F9 cell lysates with Rabbit anti-ERK1 antibody (ER131011) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER131011) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.