ER1510-11

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 54 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within human Glut-1 aa 15-60/492.

Positive control:

Human placenta tissue lysates, Hela, human liver tissue, human breast tissue, mouse liver tissue.

Subcellular location:

Cell membrane, Melanosome.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:100-1:500
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: P11166 Human | P17809 Mouse

Alternative names:

Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) CSE DYT17 DYT18 DYT9 EIG12 erythrocyte/brain Erythrocyte/hepatoma glucose transporter facilitated glucose transporter member 1 Glucose transporter 1 Glucose transporter type 1 Glucose transporter type 1, erythrocyte/brain GLUT GLUT-1 GLUT1 GLUT1DS GLUTB GT1 GTG1 Gtg3 GTR1_HUMAN HepG2 glucose transporter HTLVR Human T cell leukemia virus (I and II) receptor MGC141895 MGC141896 PED RATGTG1 Receptor for HTLV 1 and HTLV 2 SLC2A1 Solute carrier family 2 (facilitated glucose transporter), member 1 Solute carrier family 2 Solute carrier family 2, facilitated glucose transporter member 1

	Fig1: Western blot analysis of Glut1 on human placenta tissue lysates using anti-Glut1 antibody at 1/1,000 dilution.
	Fig2: ICC staining Glut1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Glut1 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. Counter stained with hematoxylin.
	Fig6: Flow cytometric analysis of Hela cells with Glut1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.