Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 237 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Dysferlin aa 124-317 / 2,080. |
Positive control: | Human fetal skeletal muscle tissue lysate, human kidney tissue, human placenta tissue, human fetal skeletal muscle tissue, HUVEC. |
Subcellular location: | Plasma membrane, Cytoplasmic vesicle. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: O75923 Human |
Alternative names: | DMAT DYSF DYSF_HUMAN Dysferlin Dysferlin limb girdle muscular dystrophy 2B (autosomal recessive) Dysferlin limb girdle muscular dystrophy 2B Dystrophy associated fer 1 like 1 Dystrophy associated fer 1 like protein Dystrophy associated fer1 like 1 Dystrophy associated fer1 like protein Dystrophy-associated fer-1-like protein Fer 1 like protein 1 Fer-1-like protein 1 Fer1 like protein 1 FER1L1 FLJ00175 FLJ90168 LGMD 2B LGMD2B Limb girdle muscular dystrophy 2B (autosomal recessive) Limb girdle muscular dystrophy 2B Miyoshi myopathy MM MMD1 |
Fig1: Western blot analysis of Dysferlin on human fetal skeletal muscle tissue lysate using anti-Dysferlin antibody at 1/500 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Dysferlin antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Dysferlin antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Dysferlin antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of HUVEC cells with Dysferlin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |