NOX2/gp91phox Rabbit Polyclonal Antibody
cat.: ER1511-34
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to C-terminal human NOX2/gp91phox. |
| Positive control: | HepG2, MCF-7, Hela, human liver tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P04839 Human | Q61093 Mouse |
| Alternative names: | AMCBX2 CGD CGD91-phox CY24B_HUMAN CYBB Cytochrome b 245, beta polypeptide Cytochrome b(558) beta chain Cytochrome b(558) subunit beta Cytochrome b-245 heavy chain Cytochrome b558 subunit beta GP91 PHOX gp91-1 gp91-phox GP91PHOX Heme-binding membrane glycoprotein gp91phox NADPH oxidase 2 Neutrophil cytochrome b 91 kDa polypeptide NOX2 p22 phagocyte B-cytochrome P91 PHOX p91-PHOX Superoxide-generating NADPH oxidase heavy chain subunit |
Images
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Fig1: ICC staining NOX2/gp91phox in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining NOX2/gp91phox in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NOX2/gp91phox in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.