NOX2/gp91phox Rabbit Polyclonal Antibody
cat.: ER1511-34
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to C-terminal human NOX2/gp91phox.
Positive control: HepG2, MCF-7, Hela, human liver tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  ICC
  IHC-P
  FC

1:50-1:200
1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P04839 Human | Q61093 Mouse
Alternative names: AMCBX2 CGD CGD91-phox CY24B_HUMAN CYBB Cytochrome b 245, beta polypeptide Cytochrome b(558) beta chain Cytochrome b(558) subunit beta Cytochrome b-245 heavy chain Cytochrome b558 subunit beta GP91 PHOX gp91-1 gp91-phox GP91PHOX Heme-binding membrane glycoprotein gp91phox NADPH oxidase 2 Neutrophil cytochrome b 91 kDa polypeptide NOX2 p22 phagocyte B-cytochrome P91 PHOX p91-PHOX Superoxide-generating NADPH oxidase heavy chain subunit
Images
ER1511-34_1.jpg Fig1: ICC staining NOX2/gp91phox in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-34_2.jpg Fig2: ICC staining NOX2/gp91phox in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-34_3.jpg Fig3: ICC staining NOX2/gp91phox in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-34_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
ER1511-34_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
ER1511-34_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
ER1511-34_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOX2/gp91phox antibody. Counter stained with hematoxylin.
ER1511-34_8.jpg Fig8: Flow cytometric analysis of Hela cells with NOX2/gp91phox antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.