WNT2 Rabbit Polyclonal Antibody
cat.: ER1511-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human WNT2 aa 209-263. |
| Positive control: | PC-3M cell lysate, Mouse brain tissue, Jurkat cell lysate, Human lung tissue, Jurkat. |
| Subcellular location: | Secreted, Extracellular matrix. |
| Recommended Dilutions:
WB FC |
1:1,000-1:2,000 1:100-1:200 |
| Uniprot #: | SwissProt: P09544 Human |
| Alternative names: | Int 1 related protein Int-1-like protein 1 Int-1-related protein INT1L 1 INT1L1 IRP IRP protein ONCOGENE INT1 LIKE 1 Protein Wnt-2 Secreted growth factor Wingless type MMTV integration site family member 2 WNT 2 WNT2 WNT2_HUMAN |
Images
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Fig1:
Western blot analysis of WNT2 on different lysates with Rabbit anti-WNT2 antibody (ER1511-4) at 1/2,000 dilution. Lane 1: MCF7 (Human breast cancer cell) cell lysate Lane 2: Jurkat(Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1511-4, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 40 kDa Observed band size: 40 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.