GRP94 Rabbit Polyclonal Antibody
cat.: ER1511-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 92 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GRP94 aa 194-247.
Positive control: HepG2 cell lysate, HeLa cell lysate, human brain tissue lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat placenta tissue lysate, rat brain tissue lysate, Hela, HepG2, MCF-7, human liver tissue, human breast tissue, human stomach cancer tissue, mouse stomach tissue.
Subcellular location: Endoplasmic reticulum lumen, Melanosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:100-1:500
1:100-1:500
Uniprot #: SwissProt: P14625 Human | P08113 Mouse | Q66HD0 Rat
Alternative names: 94 kDa glucose regulated protein 94 kDa glucose-regulated protein ECGP Endoplasmin Endothelial cell (HBMEC) glycoprotein ENPL_HUMAN Glucose regulated protein 94kDa gp96 gp96 homolog GRP 94 GRP-94 Heat shock protein 90 kDa beta member 1 heat shock protein 90kDa beta (Grp94), member 1 Heat shock protein, 90 kDa, beta, 1 HSP90B1 Stress inducible tumor rejection antigen GP96 TRA1 tumor rejection antigen (gp96) 1 Tumor rejection antigen 1 Tumor rejection antigen gp96 Tumor rejection antigen-1 (gp96)
Images
ER1511-5_1.jpg Fig1: Western blot analysis of GRP94 on different lysates with Rabbit anti-GRP94 antibody (ER1511-5) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: Mouse placenta tissue lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: Rat placenta tissue lysate (20 µg/Lane)
Lane 9: Rat brain tissue lysate (20 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 94 kDa

Exposure time: 25 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1511-5) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1511-5_2.jpg Fig2: ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_3.jpg Fig3: ICC staining GRP94 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_4.jpg Fig4: ICC staining GRP94 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.