GRP94 Rabbit Polyclonal Antibody
cat.: ER1511-5
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 94 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human GRP94 aa 194-247.
Positive control: HepG2, NIH-3T3, PC-12, Hela, MCF-7, human liver tissue, human stomach cancer, human breast tissue, mouse stomach tissue.
Subcellular location: Endoplasmic reticulum lumen. Melanosome.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:100-1:500
1:100-1:500
Uniprot #: SwissProt: P14625 Human | P08113 Mouse | Q66HD0 Rat
Alternative names: 94 kDa glucose regulated protein 94 kDa glucose-regulated protein ECGP Endoplasmin Endothelial cell (HBMEC) glycoprotein ENPL_HUMAN Glucose regulated protein 94kDa gp96 gp96 homolog GRP 94 GRP-94 Heat shock protein 90 kDa beta member 1 heat shock protein 90kDa beta (Grp94), member 1 Heat shock protein, 90 kDa, beta, 1 HSP90B1 Stress inducible tumor rejection antigen GP96 TRA1 tumor rejection antigen (gp96) 1 Tumor rejection antigen 1 Tumor rejection antigen gp96 Tumor rejection antigen-1 (gp96)
Images
ER1511-5_1.jpg Fig1: Western blot analysis of GRP94 on different cell lysate using anti-GRP94 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: NIH-3T3
Lane 3: PC-12
ER1511-5_2.jpg Fig2: ICC staining GRP94 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_3.jpg Fig3: ICC staining GRP94 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_4.jpg Fig4: ICC staining GRP94 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1511-5_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
ER1511-5_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GRP94 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.