DKK1 Rabbit Polyclonal Antibody
cat.: ER1512-20
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human DKK1. |
| Positive control: | Mouse brain tissue lysate, human fetal brain tissue lysate, Hela, NCCIT, human placenta tissue, mouse placenta tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O94907 Human | O54908 Mouse |
| Alternative names: | Dickkopf 1 Dickkopf 1 homolog Dickkopf 1 like Dickkopf homolog 1 Dickkopf like protein 1 Dickkopf related protein 1 Dickkopf WNT signaling pathway inhibitor 1 Dickkopf-1 Dickkopf-related protein 1 DKK 1 Dkk-1 Dkk1 DKK1_HUMAN hDkk 1 hDkk-1 SK |
Images
|
Fig1:
Western blot analysis of DKK1 on different tissue lysates using anti-DKK1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue lysate Lane 2: Human fetal brain tissue lysate |
|
Fig2: ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-DKK1 antibody. Counter stained with hematoxylin. |
|
Fig6: Flow cytometric analysis of Hela cells with DKK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.